Monitoring Lake Water

Water Testing (Addendum contains sampling protocol, data sheets)
Culver Lake water needs to be monitored on a routine schedule during the growing season that begins in early April and continues into November. The data collected is used by lake management to assess the changing conditions of the lake’s ecology which responds to the environment. These data are distributed to all parties that make recommendations and conduct lake management activities.

Approximately once a week from April through November, data consisting of DO and temperature in degrees Celsius every 1 meter from surface to 14 meters and secchi disc clarity are recorded consistent with weather and boat availability. These data are recorded on the data sheet (see addendum) at 900 feet north location. This approximate location is used each year is a spot about 900 feet off the clubhouse dock at an intersection of two lines from the Clubhouse to Okesens and Gibles to Baldassono’s houses. GPS coordinates 41deg. 10 min.14.56 sec. N, 74deg. 46 min 25.47 sec. W provide a more accurate method of location of 900N.
Procedure Check List (recommend minimum two people)

Weekly Monitoring
Volunteers do monitoring in early spring and late fall. Trained patrol personnel will do monitoring when their schedule permits. It is always beneficial to have a water quality volunteer aboard. It is best to take readings in mid-late morning while sun is high and other boating is minimal.

Equipment: D.O./Temp. YSI meter, Secchi disc., data sheet and pen.

Obtain keys for Patrol Boat from closet between office and meeting room.

Ready boat and cruise to and anchor at 900 north. Turn on YSI and allow warm-up for 10 minutes. Calibrate YSI before use at this time before immersing in water. Use zero salinity, 800 ft. altitude

Uncoil cable carefully.

Take a secchi reading -two people either side of boat after boat is held by anchor. Record in tenths of a meter. This requires some estimation between marks on rope. Red marks are whole meters and black marks are half meter.

Begin at surface and take reading of T and DO. Gently move probe in water and take reading after temperature reading is stable.

Continue at one meter intervals down to 14 meters. The cable is marked by bands of red tape at each meter. The 14 meter reading will require moving the meter near the gunwale as the cable is short at this depth.

Wildco Depth Sampler (top), Secci Disk (middle), YSI D.O./Temp. Monitor (right)

After all readings are recorded , carefully recoil cable on holder, rinse the probe with distilled water from drinking water bottle, add a few drops inside storage chamber to keep sponge moist, and reinsert in storage chamber in back of meter.

Note on data sheet for current weather and recent rainfall and who took data.

Haul up anchor and cruise to Stehr Cove for weed inspection and note growth and specie types. In midsummer, check areas that were treated with herbicide.

Total time about 1 hour

Dock boat on lifter and return keys.

Uploading Data
Open the Excel sheet from last data and do a “save as” by updating the file name to the current date.

Over write the new data over the previous data for each meter’s DO and temp. Also convert Secchi reading from meters to feet and over write the last data. (Meters x 3.3 ft/meter)

Do a save and attach to email to Water readings distribution list and a bcc to WQ members. Note any unusual conditions such as recent rain fall, weed conditions, aerator units operating and air flows.

The water readings distribution list includes consultants and herbicide contractors

Sampling Procedure/Equipment

The schedule for sampling is shown in the Addendum. The sampling requires familiarity and understanding of the proper procedure for water samples and biology samples. Only trained personnel are to sample.

Water samples are taken at 8 monthly intervals for all stakeholders in the lake management special interest group. The sampling data, reported by laboratory electronically, will be distributed via email attachment to a distribution list. This includes: our consultants, E.S.C (Dr. Robert Kortmann), Princeton Hydro (Dr. Steven Souza, Chris. M.), Normanoch Water Readings Distribution list that includes Lake management Directors and members of the Water Quality Committee. The data from the lab will be kept in our website library.

Sampling intervals are mid-month of April through November. Refer to addendum for protocol for each month sampling. Four water samples are taken at 1m, 4m, 8m. and 12m. One 5m zooplankton sample and one 5m algae draw sample. Bottle sizes are determined by what the laboratory provides. All water samples destined to the laboratory will be conveyed by chain of custody. This requires a signature of responsible party at time of sample pickup.

Generally we take the previously described water readings before doing the 6 samples. So you will need, data sheet, permanent marking pencil, lugorol preservative, 6 sample bottles and the following equipment: the YSI 550A DO/temp. Meter, Secchi Disc, Wildco Water Sampler, the 5 meter algae tubing, and the Wildco Zooplankton sampling net. These are in the WQ equipment

Water Sampling Procedure (this should be done on Wednesday but no later than Thursday to allow for samples pickup by the Lab.). If samples are taken on the weekend, they must be refrigerated until pick up .

Pre-label the sample bottles and stickers in advance. Each week may have a different sample protocol so follow sample table.

Anchor at the 900N location as described above.

Record the water DO and temperature readings along with secchi disc reading as described previously.

Set the sampler (small valves closed and ends open) and lower the water sampler to 1 meter depth as indicated by the red mark on the rope.

Drop the weight and pull up the sampler and drain water sample into 500 ml. bottle labeled 1 meter. Fill sample bottle and overflow so that there is no air. Make bottle is properly labeled and agrees with Lab sticker.

Do the same for the other 3 water samples. Make sure tops are on tight.

Algae Sample Procedure
Lower the clear polypropylene hose weight down into water all the way with about 4 feet of tube above the water surface.

Double over the end and place your finger over the exposed end to prevent air leaking into hose, breaking vacuum and losing sample.

Withdraw hose and with the assistance of a helper, place weighted end into properly labeled 250 ml. bottle and allow air to enter hose. Gradually work all the water out of hose. There will be a small sample of water in bottle – perhaps 25-50 ml. is sufficient. Add 3 drops of Lugurol and tighten down top. Be careful handling the Lugurol that you don’t get any on your skin or clothing. It is iodine and it will stain.

Zooplankton Sample Procedure
Before lowering the Zoo. Net, loosen plug on bottom of sample container and reinsert plug medium firmly.

Lower the Zooplankton sampler to 5 meters depth.

Pull the net upward continuously and as soon as it emerges, with assistance of someone holding container, remove plug. Allow contents to drain in marked 250 ml. bottle. Look inside and see if you can see Zoos. Swimming around. Add 3 drops of Lugorol and seal bottle. Carefully store Lugorol in equipment box.

End of sampling.

Storage and Shipment of samples
Each bottle must be labeled and placed in a special shipping container provided by the laboratory. The chain of custody form must accompany the sample shipment.

Cyano bacteria surveillance